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020 _a9781592598120
024 7 _a10.1385/1592598129
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aApoptosis Methods and Protocols
_cedited by Hugh J. M. Brady.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XI, 304 páginas)
_b74 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v282
505 0 _aMeasurement of Apoptosis by DNA Fragmentation -- Measurement of Caspase Activity in Cells Undergoing Apoptosis -- Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells -- Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays -- Detecting and Quantifying Apoptosis in Tissue Sections -- Immunoassay for Single-Stranded DNA in Apoptotic Cells -- Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptosis -- Measurement of Changes in Intracellular Calcium During Apoptosis -- Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2 -- Assays to Measure Stress-Activated MAPK Activity -- Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability -- Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons -- Measuring Programmed Cell Death in Plants -- Detection of Apoptosis in Drosophila -- Measurement of Apoptotic Cell Clearance In Vitro -- Yeast Two-Hybrid Screening for Proteins Interacting With the Anti-Apoptotic Protein A20 -- The Yeast Three-Hybrid System As a Tool to Study Caspases -- Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA -- Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis.
520 _aThe ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.
700 1 _aBrady, Hugh J. M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489939562
776 0 8 _iPrinted edition:
_z9781489939555
776 0 8 _iPrinted edition:
_z9780896038738
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598129
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c235487
_d235487