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020 _a9781617790997
024 7 _a10.1007/978-1-61779-099-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMammalian Chromosome Engineering
_bMethods and Protocols
_cedited by Gyula Hadlaczky.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (IX, 257 páginas)
_b48 ilustraciones, 3 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v738
505 0 _aDeveloping Extrachromosomal Gene Expression Vector Technologies: An Overview -- High Capacity Extrachromosomal Gene Expression Vectors -- Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview -- Chromosome Transfer Via Cell Fusion -- Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview -- Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System -- Construction and Use of a Bottom-Up HAC Vector for Transgene Expression -- De novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification -- Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes -- Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System -- Dendrimer Mediated Transfer of Engineered Chromosomes -- Engineered Chromosomes in Transgenics -- Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System -- Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview -- Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects.
520 _aThe rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.  Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.  Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.
700 1 _aHadlaczky, Gyula
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617790980
776 0 8 _iPrinted edition:
_z9781617791000
776 0 8 _iPrinted edition:
_z9781493957750
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-099-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c235488
_d235488