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020 _a9781592590605
024 7 _a10.1385/1592590608
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aNeurotrophin Protocols
_cedited by Robert A. Rush.
250 _a1st edition 2001
264 1 _aTotowa, NJ
_bHumana Press
_c2001
300 _a1 recurso en línea (X, 274 páginas)
_b41 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v169
505 0 _aNeurotrophin Quantification and Localization -- Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistry -- Neurotrophin Immunohistochemistry in Peripheral Tissues -- Extraction and Quantification of the Neurotrophins -- Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques -- RNA Quantification and Localization -- Quantification of mRNA Levels Using Northern Blotting -- Quantification of mRNA Levels Using Ribonuclease Protection Assay -- Quantification of Neurotrophin mRNA by RT-PCR -- Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System -- Construction of Recombinant Proteins,and Transgenic Cells and Animals -- Expression of Neurotrophic Factors such as GDNF in the E. coli System -- Construction of Cells Expressing Neurotrophins -- Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC) -- Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins -- Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors -- In Vivo Analysis -- Tracing with Radiolabeled Neurotrophins -- Toxin-Induced Death of Neurotrophin-Sensitive Neurons -- Oligonucleotides as Inhibitors of Protein Synthesis -- The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins -- Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination.
520 _aDespite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system.
700 1 _aRush, Robert A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489939838
776 0 8 _iPrinted edition:
_z9781489939821
776 0 8 _iPrinted edition:
_z9780896036994
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590608
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2001
999 _c235490
_d235490