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020 _a9781592597628
024 7 _a10.1385/1592597629
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aProtein'Protein Interactions
_bMethods and Applications
_cedited by Haian Fu.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XVI, 532 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v261
505 0 _aOverview -- Structural Basis of Protein-Protein Interactions -- Quantitative Analysis of Protein-Protein Interactions -- In Vitro Techniques -- Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry -- Circular Dichroism Analysis for Protein-Protein Interactions -- Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance -- Using Light Scattering to Determine the Stoichiometry of Protein Complexes -- Sedimentation Equilibrium Studies -- Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography -- Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions -- Fluorescence Polarization Assay to Quantify Protein-Protein Interactions -- Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot -- Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions -- Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery -- Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting -- Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions -- Detecting Protein-Protein Interactions in Heterologous Systems -- A Bacterial Two-Hybrid System Based on Transcription Activation -- Using the Yeast Two-Hybrid System to Identify Interacting Proteins -- Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System -- The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System -- Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo -- Co-Immunoprecipitation from Transfected Cells -- Probing Protein-Protein Interactions in Living Cells -- Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET) -- Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer -- Fluorescence Correlation Spectroscopy -- Confocal Microscopy for Intracellular Co-Localization of Proteins -- Mapping Biochemical Networks With Protein-Fragment Complementation Assays -- In Vivo Protein Cross-Linking -- Proteomics-Based Approaches -- Computational Prediction of Protein-Protein Interactions -- Affinity Methods for Phosphorylation-Dependent Interactions -- Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes -- Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry -- Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry -- Internet Resources for Studying Protein-Protein Interactions.
520 _aProteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets.
700 1 _aFu, Haian
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617373732
776 0 8 _iPrinted edition:
_z9781489939685
776 0 8 _iPrinted edition:
_z9781588291202
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597629
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c235493
_d235493