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001			235494
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007			cr nn 008mamaa
008			100301s1996 xxu| s |||| 0|eng d
020			_a9781592595433
024	7		_a10.1385/0896032590 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aJones, Christopher. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aCrystallographic Methods and Protocols _cby Christopher Jones, Barbara Mulloy, Mark R. Sanderson.
250			_a1st edition 1996
264		1	_aTotowa, NJ _bHumana Press _c1996
300			_a1 recurso en línea (408 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v56
505	0		_aOverexpression, Isolation, and Crystallization of Proteins -- Preliminary Characterization of Crystals -- Modern Methods for Rapid X-Ray Diffraction Data Collection from Crystals of Macromolecules -- Use of Multiple-Wavelength Anomalous Diffraction Measurements in Ab Initio Phase Determination for Macromolecular Structures -- Structure Determination Using Isomorphous Replacement -- Molecular Replacement Using Known Structural Information -- Density Modification in X-Ray Crystallography -- Refinement of Protein and Nucleic Acid Structures -- Recent Developments for Crystallographic Refinement of Macromolecules -- The Crystallization and Structure Analysis of Oligonucleotide Sequences -- Crystallography in the Study of Protein-DNA Interactions -- Virus Crystallography -- Crystallization and Structure Analysis of Membrane Proteins.
520			_aThe volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three­ dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable resources. The biologically trained worker will probably first become involved when identifying an important scientific problem whose solution would benefit from a full structure. The protein or nucleic acid at issue must be sequenced and prepared to high purity in appropriate quantities, probably by either chemical synthesis for nucleic acids or genetic engineering for proteins. CrystallographicMethods andPro­ tocols aims to give biologically trained workers an insight into the techniques used to crystallize their proteins, obtain the raw X-ray data, and solve and refine the structure. The aim ofa crystal structure determination is to provide infor­ mation that will solve biologically relevant problems; that process normally requires a high resolution structure. The preparationofsuit­ able crystals for a high resolution structure remains the major bottle­ neck in structure determination, and the effective application of appropriate genetic engineering and biochemical techniques in the initial stages pays ahandsome dividend later. The productionofsuit­ able proteins by recombinant methods and the preparationofcrystal­ line derivatives are covered by Skelly and Madden in Chapter 2. Before beginning the data collection required for a high resolution structure, a preliminary characterizationofthe crystals is carried out.
700	1		_aMulloy, Barbara. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
700	1		_aSanderson, Mark R. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0	8	_iPrinted edition: _z9781489940438
776	0	8	_iPrinted edition: _z9780896032590
776	0	8	_iPrinted edition: _z9781489940421
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032590 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1996
999			_c235494 _d235494