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001			235496
003			ES-VaUE
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007			cr nn 008mamaa
008			110923s2012 xxu| s |||| 0|eng d
020			_a9781617793400
024	7		_a10.1007/978-1-61779-340-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aOncolytic Viruses _bMethods and Protocols _cedited by David H. Kirn, Ta-Chiang Liu, Stephen H. Thorne.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (X, 241 páginas) _b35 ilustraciones, 11 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v797
505	0		_aOncolytic Herpes Simplex Virus Engineering and Preparation -- Construction of Capsid-modified Adenoviruses by Recombination in Yeast and Purification by Iodixanol-gradient -- Construction of Targeted and Armed Oncolytic Adenoviruses -- Syrian Hamster Tumor Model to Study Oncolytic Ad5 Based Vectors -- Adenoviral Gene Expression and Replication in Human Tumor Explant Models -- Imaging Luciferase-expressing Viruses -- In vivo Positron Emission Tomography Imaging using the Sodium Iodide Symporter as a Reporter Gene -- Maintaining and Loading Neural Stem Cells for Delivery of Oncolytic Adenovirus to Brain Tumors -- Targeting Brain Tumor Stem Cells with Oncolytic Adenoviruses -- Propagation, Purification and In vivo Testing of Oncolytic Vesicular Stomatitis Virus Strains -- Oncolytic Measles Virus Retargeting by Ligand Display -- Exploring Host Factors that Impact Reovirus Replication, Dissemination, and Reovirus-induced Cell Death in Cancer versus Normal Cells in Culture -- Analysis of Three Properties of Newcastle Disease Virus for Fighting Cancer: Tumor Selective Replication, Antitumor Cytotoxicity and Immunostimulation -- Next Generation Oncolytic Vaccinia Vectors -- Evaluation of Innate Immune Signaling Pathways in Transformed Cells.
520			_aSince the first report of an engineered oncolytic virus, there has been a continuing and steady increase of interest in the field, and while bench research remains vital for the translation of research in this field, its success depends on breakthroughs in clinical studies. Oncolytic Viruses: Methods and Protocols describes the construction and purification of capsid-modified adenoviruses as well as oncolytic adenoviruses, presents protocols for many individual virus species including engineering and preparation of oncolytic HSV, propagation, purification, and in vivo testing of oncolytic VSV, details properties of oncolytic reovirus and NDV, and describes the generation and testing of next generation of oncolytic vaccinia virus. As the host immune system plays a critical role in determining efficacy of oncolytic viruses, two chapters are devoted to the study of immune response. Recent advances in stem cell research have led the field in two distinct directions: the use of stem cells as carrier vehicles for oncolytic viruses and the targeting of cancer stem cells. As such, the volume describes the use of explant tissue samples from patients to potentially provide useful information predicting responses prior to clinical translation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Oncolytic Viruses: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential and vital field.
700	1		_aKirn, David H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLiu, Ta-Chiang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aThorne, Stephen H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617793394
776	0	8	_iPrinted edition: _z9781617793417
776	0	8	_iPrinted edition: _z9781493958122
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-340-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c235496 _d235496