000			03642nam a22003615i 4500
001			235499
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592595624
024	7		_a10.1385/0896033554 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGlycoanalysis Protocols _cedited by Elizabeth F. Hounsell.
250			_a2nd edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (X, 262 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v76
505	0		_aCharacterization of Protein Glycosylation -- Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides -- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography -- Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM) -- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column -- HPLC and HPAEC of Oligosaccharides and Glycopeptides -- Analysis of N- and O-Glycans by Pyridylamination -- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins -- Analysis of Glycosaminoglycans and Proteoglycans -- The Use of Cell and Organ Culture for the Study of Secreted Mucins -- Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides -- TLC in Structure and Recognition Studies of Glycosphingolipids -- Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids -- Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures -- Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.
520			_aNow fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.
700	1		_aHounsell, Elizabeth F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617370168
776	0	8	_iPrinted edition: _z9780896033559
776	0	8	_iPrinted edition: _z9781489942616
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033554 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c235499 _d235499