000			04437nam a22003615i 4500
001			235500
003			ES-VaUE
005			20221220020709.0
007			cr nn 008mamaa
008			151119s2016 xxu| s |||| 0|eng d
020			_a9781493930494
024	7		_a10.1007/978-1-4939-3049-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPhospho-Proteomics _bMethods and Protocols _cedited by Louise von Stechow.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing : _bImprint: Springer, _c2016
300			_a1 recurso en línea (XIII, 364 páginas) _b61 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1355
505	0		_aThiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins -- Phosphopeptide Detection with Biotin-Labeled Phos-tag -- Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports -- Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics -- Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles -- Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM) -- Enrichment Strategies in Phosphoproteomics -- Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography -- The use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides -- Sequential Elution from IMAC (SIMAC) - An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated Peptides -- Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation -- Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage -- Phosphopeptide Enrichment Using Various Magnetic Nanocomposites - An Overview -- Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics -- Variable Digestion Strategies for Phosphoproteomics Analysis -- On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins -- Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity -- Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics -- Phosphoprotein Detection by High-Throughput Flow Cytometry -- Resources for Assignment of Phosphorylation Sites on Peptides and Proteins -- From Phosphosites to Kinases -- Search Databases and Statistics - Pitfalls and Best Practices in Phosphoproteomics -- Systems Analysis for Interpretation of Phosphoproteomics Data.
520			_aThis second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phospho-Proteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Phospho-Proteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
700	1		_avon Stechow, Louise _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493930487
776	0	8	_iPrinted edition: _z9781493930500
776	0	8	_iPrinted edition: _z9781493950010
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3049-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235500 _d235500