000			04482nam a22003735i 4500
001			235508
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007			cr nn 008mamaa
008			140607s2014 xxu| s |||| 0|eng d
020			_a9781493909223
024	7		_a10.1007/978-1-4939-0922-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant MAP Kinases _bMethods and Protocols _cedited by George Komis, Jozef Šamaj.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 266 páginas) _b33 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1171
505	0		_aTransient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling -- Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR -- Analysis of MAPK Activities using MAPK-specific Antibodies -- Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels -- Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™ -- Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae -- Virus-induced Gene Silencing in Plant MAPK Research -- RNA Interference of Plant MAPK Cascades for Functional Studies -- Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes -- Immunofluorescent Localization of MAPKs in Steedman's Wax Sections -- Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies -- Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases -- Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1 -- In vivo Phosphorylation of WRKY Transcription Factor by MAPK -- Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates -- Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome -- Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays.
520			_aMitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.
700	1		_aKomis, George _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aŠamaj, Jozef _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493909230
776	0	8	_iPrinted edition: _z9781493909216
776	0	8	_iPrinted edition: _z9781493945894
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0922-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235508 _d235508