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001			235509
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008			150319s2015 xxu| s |||| 0|eng d
020			_a9781493925476
024	7		_a10.1007/978-1-4939-2547-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSmall Non-Coding RNAs _bMethods and Protocols _cedited by Mathieu Rederstorff.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 238 páginas) _b35 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1296
505	0		_aSmall Non-Coding RNAs: A Quick Look in the Rear-View Mirror -- Alcoholic Precipitation of Small Non-Coding RNAs -- Quantification and Quality Control of a Small Non-Coding RNA Preparation -- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting -- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes -- Direct Cloning of Double Stranded RNAs -- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation -- Fluorescence in situ Hybridization of Small Non-Coding RNAs -- RT-qPCR Based Quantification of Small Non-Coding RNAs -- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs -- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs -- Probing Small Non-Coding RNAs Structures -- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing -- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells -- Microarray Analysis of Small Non-Coding RNAs -- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes -- Dual Luciferase Gene Reporter Assays to Study miRNA Function -- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells -- Efficient and Selective Knockdown of Small Non-Coding RNAs -- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands -- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.
520			_aThis volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology.  Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular, and molecular biologists already working in the field of RNA biology  and to those just starting to study small non-coding RNAs.
700	1		_aRederstorff, Mathieu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493925483
776	0	8	_iPrinted edition: _z9781493925469
776	0	8	_iPrinted edition: _z9781493949038
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2547-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235509 _d235509