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020 _a9781493969586
024 7 _a10.1007/978-1-4939-6958-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMicrobial Toxins
_bMethods and Protocols
_cedited by Otto Holst.
250 _a2nd edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XI, 201 páginas)
_b71 ilustraciones, 28 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1600
505 0 _aDetection of Cholera Toxin by an Immunochromatographic Test Strip -- Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins -- A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis -- Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin -- Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food -- Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection -- Detection of Yersinia pestis in Complex Matrices by Intact Cell Immunocapture and Targeted Mass Spectrometry -- A Method to Prepare Magnetic Nanosilicate Platelets for Effective Removal of Microcystis aeruginosa and Microcystin LR -- An Immunochromatographic Test Strip to Detect Ochratoxin A and Zearalenone Simultaneously -- Endotoxin Removal from Escherichia coli Bacterial Lysate using Biphasic Liquid System -- Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins -- Laser Interferometry Method as a Novel Tool in Endotoxin Research -- Endotoxin Entrapment on Glass via C18 Self-Assembled Monolayers and Rapid Detection using Drug-Nanoparticle Bioconjugate Probes -- A Bioassay for Determination of Lipopolysaccharides and Lipoproteins -- Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes and Subclasses -- Micromethods for Isolation and Structural Characterization of Lipid A, and Polysaccharide Regions of Bacterial Lipopolysaccharides -- Mass Spectrometry for Profiling LOS and Lipid A Structures from Whole-Cell Lysates: Directly from a Few Bacterial Colonies or from Liquid Broth Cultures.
520 _aThis second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.
700 1 _aHolst, Otto
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493969562
776 0 8 _iPrinted edition:
_z9781493969579
776 0 8 _iPrinted edition:
_z9781493983513
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6958-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c235510
_d235510