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020 _a9781493972258
024 7 _a10.1007/978-1-4939-7225-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPhotorespiration
_bMethods and Protocols
_cedited by Alisdair R. Fernie, Hermann Bauwe, Andreas P.M. Weber.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XIV, 274 páginas)
_b40 ilustraciones, 25 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1653
505 0 _aEstimation of Photorespiratory Fluxes by Gas Exchange -- Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling -- Measurement of Enzyme Activities -- In vitro Alkylation Methods for Assessing the Protein Redox State -- Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants -- In vitro Analysis of Metabolite Transport Proteins -- Quantification of Photorespiratory Intermediates by Mass Spectrometry-based Approaches -- Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration -- Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits -- Metabolic Engineering of Photorespiration -- 13CO2 Labelling and Mass Spectral Analysis of Photorespiration -- Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants -- Genome-scale Modeling of Photorespiratory Pathway Manipulation -- Kinetic Modelling of Photorespiration -- Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues -- Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana -- Light Microscopy, Transmission Electron Microscopy and Immunohistochemistry Protocols for Studying Photorespiration. .
520 _aThis volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700 1 _aFernie, Alisdair R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBauwe, Hermann
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWeber, Andreas P.M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493972241
776 0 8 _iPrinted edition:
_z9781493972265
776 0 8 _iPrinted edition:
_z9781493984220
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7225-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c235518
_d235518