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001			235529
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007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781603271646
024	7		_a10.1007/978-1-60327-164-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacteriophages _bMethods and Protocols, Volume 1: Isolation, Characterization, and Interactions _cedited by Martha R. J. Clokie, Andrew Kropinski.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XXII, 308 páginas) _b41 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v501
505	0		_aBacteriophage Isolation -- Methods for the Isolation of Viruses from Environmental Samples -- Bacteriophage Enrichment from Water and Soil -- Isolation of Phage via Induction of Lysogens -- Isolation of Cyanophages from Aquatic Environments -- Isolation of Viruses from High Temperature Environments -- Isolation of Novel Large and Aggregating Bacteriophages -- Bacteriophage Characterization -- Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay -- Enumeration of Bacteriophages by the Direct Plating Plaque Assay -- Enumeration of Bacteriophages Using the Small Drop Plaque Assay System -- Determination of Virus Abundance by Epifluorescence Microscopy -- Enumeration of Bacteriophages Using Flow Cytometry -- Basic Phage Electron Microscopy -- Phage Classification and Characterization -- Phage Host Range and Efficiency of Plating -- Measurement of the Rate of Attachment of Bacteriophage to Cells -- Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors -- Bacteriophage Plaques: Theory and Analysis -- Practical Methods for Determining Phage Growth Parameters -- Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes -- Bacteriophage-Host Interactions -- Construction of Phage Mutants -- Modifying Bacteriophage with Recombineering -- Identification and Isolation of Lysogens with Induced Prophage -- Generalized Transduction -- Preparation and Characterization of Anti-phage Serum -- Generalized Transduction by Lytic Bacteriophages.
520			_aRanging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
700	1		_aClokie, Martha R. J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKropinski, Andrew _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607610984
776	0	8	_iPrinted edition: _z9781617377150
776	0	8	_iPrinted edition: _z9781588296825
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-164-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235529 _d235529