000			03241nam a22003615i 4500
001			235536
003			ES-VaUE
005			20221220020711.0
007			cr nn 008mamaa
008			170622s2017 xxu| s |||| 0|eng d
020			_a9781493971282
024	7		_a10.1007/978-1-4939-7128-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGenome Editing in Animals _bMethods and Protocols _cedited by Izuho Hatada.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 256 páginas) _b67 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1630
505	0		_aConstruction and Evaluation of Zinc Finger Nucleases -- Current Overview of TALEN Construction Systems -- CRISPR/Cas9 -- Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse -- Genome Editing of Mouse by Cytoplasmic Injection -- Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids -- Genome Editing in Mouse and Rat by Electroporation -- Generation of Knock-in Mouse by Genome Editing -- Genome Editing of Rat -- Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids -- Genome Editing of Pig -- Genome Editing of Monkey -- Genome Editing Mediated by Primordial Germ Cell in Chicken -- CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish -- Genome Editing of Medaka -- A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System -- Genome Editing of Silkworms -- Genome Editing in the Cricket, Gryllus bimaculatus -- Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease -- Genome editing of C. elegans.
520			_aThis volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aHatada, Izuho _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971275
776	0	8	_iPrinted edition: _z9781493971299
776	0	8	_iPrinted edition: _z9781493983933
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7128-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235536 _d235536