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008			160123s2016 xxu| s |||| 0|eng d
020			_a9781493933358
024	7		_a10.1007/978-1-4939-3335-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aToll-Like Receptors _bPractice and Methods _cedited by Claire McCoy.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIV, 455 páginas) _b58 ilustraciones, 29 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1390
505	0		_aToll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action -- Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures -- Toll-Like Receptor Interactions Measured by Microscopic and Flow Cytometric FRET -- Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors -- Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing -- Methods for Delivering DNA to Intracellular Receptors -- Detection of Interaction between Toll-Like Receptors and other Transmembrane Proteins by Co-Immunoprecipitation Assay -- Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity -- Measuring Monomer-to-Filament Transition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors -- Co-Transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen -- Simple Methods to Investigate MicroRNA Induction in Response to Toll-Like Receptors -- Determining the Function of Long Noncoding RNA in Innate Immunity -- Analysis of Post-Transcriptional Gene Regulation of Nod-Like Receptors via the 3'UTR -- TLR Function in Murine CD4+ T Lymphocytes and their Role in Inflammation -- Flow Cytometry Analysis of B Cell Activation and Antibody Responses Induced by Toll-Like Receptors -- Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells -- Analysis of TLR-Induced Metabolic Changes in Dendritic Cells Using the Seahorse XFe96 Extracellular Flux Analyzer -- Toll-Like Receptor Signaling and the Control of Intestinal Barrier Function -- Understanding the Role of Cellular Molecular Clocks in Controlling the Innate Immune Response -- Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis -- Allergens and Activation of the Toll-Like Receptor Response -- Investigating the Role of Toll-Like Receptors in Models of Arthritis -- Delineating the Role of Toll-Like Receptors in the Neuro-Inflammation Model EAE -- The Use of MiRNA Antagonists in the Alleviation of Inflammatory Disorders -- Investigating the Role of Toll-Like Receptors in Mouse Models of Gastric Cancer.
520			_aThis second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.
700	1		_aMcCoy, Claire _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493933334
776	0	8	_iPrinted edition: _z9781493933341
776	0	8	_iPrinted edition: _z9781493980239
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3335-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235540 _d235540