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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591817
024	7		_a10.1385/1592591817 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPosttranslational Modification of Proteins _bTools for Functional Proteomics _cedited by Christoph Kannicht.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XI, 322 páginas) _b19 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v194
505	0		_aAssignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry -- Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods -- Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) -- Separation of N-Glycans by HPLC -- Enzymatic Sequence Analysis of N-Glycans -- Immunological Detection of O-GlcNAc -- Analysis of O-Glycosylation -- Characterization of Site-Specific Glycosylation -- Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid -- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa -- Analysis of S-Acylation of Proteins -- Immunoblotting Methods for the Study of Protein Ubiquitination -- Analysis of Methylation and Acetylation in E. coli Ribosomal Proteins -- Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry -- Analysis of Tyrosine-O-Sulfation -- ?-Amidated Peptides: Approaches for Analysis -- ?-Glutamate and p-Hydroxyaspartate in Proteins -- Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides -- Lysine Hydroxylation and Crosslinking of Collagen -- Heterologous Expression in Endocrine Cells for Analysis of Posttranslational Modifications -- 2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern.
520			_aThe majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation. Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.
700	1		_aKannicht, Christoph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489938640
776	0	8	_iPrinted edition: _z9780896036789
776	0	8	_iPrinted edition: _z9781489938633
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591817 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235548 _d235548