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020 _a9781493911332
024 7 _a10.1007/978-1-4939-1133-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHematopoietic Stem Cell Protocols
_cedited by Kevin D. Bunting, Cheng-Kui Qu.
250 _a3rd edition 2014
264 1 _aNew York, NY
_bSpringer International Publishing
_c2014
300 _a1 recurso en línea (XII, 364 páginas)
_b52 ilustraciones, 42 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1185
505 0 _aThe Hematopoietic Stem Cell Landscape -- Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow -- Single-Cell PCR Profiling of Gene Expression in Hematopoiesis -- Hematopoietic Stem and Progenitor Cell Mobilization in Mice -- Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells -- Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells -- Gene Expression Profiling of Hematopoietic Stem Cells (HSC) -- Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells -- DNA Methylation Profiling of Hematopoietic Stem Cells -- Metabolic Characterization of Hematopoietic Stem Cells -- Nano Proteomic Assays on Hematopoietic Stem Cells -- Hematopoietic Differentiation of Pluripotent Stem Cells in Culture -- Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells -- Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviral Barcoding of Hematopoietic Stem Cells -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells.
520 _aUpdating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases.  An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation.
700 1 _aBunting, Kevin D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aQu, Cheng-Kui
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493911349
776 0 8 _iPrinted edition:
_z9781493911325
776 0 8 _iPrinted edition:
_z9781493947577
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1133-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2014
999 _c235550
_d235550