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001			235554
003			ES-VaUE
005			20221220020712.0
007			cr nn 008mamaa
008			130822s2013 xxu| s |||| 0|eng d
020			_a9781627035835
024	7		_a10.1007/978-1-62703-583-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMembrane Proteins _bFolding, Association, and Design _cedited by Giovanna Ghirlanda, Alessandro Senes.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 246 páginas) _b61 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1063
505	0		_aUse of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments -- Measurement of Transmembrane Peptide Interactions in Liposomes Using Förster Resonance Energy Transfer (FRET) -- Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping -- Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes -- Analyzing the Effects of Hydrophobic Mismatch on Transmembrane α-Helices Using Tryptophan Fluorescence Spectroscopy -- Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure -- Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes -- Membrane Protein Structure Determination: Back to the Membrane -- On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides -- Prediction and Design of Outer Membrane Protein-Protein Interactions -- Design of Transmembrane Peptides: Coping with Sticky Situations -- Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors -- Fluorination in the Design of Membrane Protein Assemblies.
520			_aFocusing on model systems for the study of structure, folding, and association in the membrane, Membrane Proteins: Folding, Association, and Design presents an overview of methods that can be applied to these intricate systems. The volume is divided into four detailed sections, covering association of transmembrane helices, interactions with the lipid bilayer, NMR methods, as well as a variety of engineering approaches.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Membrane Proteins: Folding, Association, and Design serves as an ideal guide for researchers reaching for the tantalizing possibility of designing novel membrane proteins with tailored functionality.
700	1		_aGhirlanda, Giovanna _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSenes, Alessandro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627035842
776	0	8	_iPrinted edition: _z9781627035828
776	0	8	_iPrinted edition: _z9781493960217
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-583-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235554 _d235554