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020 _a9781597455121
024 7 _a10.1007/978-1-59745-512-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSalmonella
_bMethods and Protocols
_cedited by Heide Schatten, Abe Eisenstark.
250 _a1st edition 2007
264 1 _aTotowa, NJ
_bHumana Press
_c2007
300 _a1 recurso en línea (XII, 364 páginas)
_b129 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v394
505 0 _aQuantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex® xMAP™ Suspension Array -- Detection of Salmonella by Bacteriophage Felix 01 -- Physical Mapping of Salmonella Genomes -- Cloth-Based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella -- Genome-Based Identification and Molecular Analyses of Pathogenicity Islands and Genomic Islands in Salmonella enterica -- Determination of the Gene Content of Salmonella Genomes by Microarray Analysis -- In Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large Bacterial DNA Segments Using VEX-Capture -- Amplified Fragment Length Polymorphism Analysis of Salmonella enterica -- Salmonella Phages and Prophages-Genomics and Practical Aspects -- Salmonella Typhimurium Phage Typing for Pathogens -- Salmonella Phages Examined in the Electron Microscope -- Applications of Cell Imaging in Salmonella Research -- Analysis of Kinesin Accumulation on Salmonella-Containing Vacuoles -- Magnesium, Manganese, and Divalent Cation Transport Assays in Intact Cells -- Methods in Cell-to-Cell Signaling in Salmonella -- Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines -- Further Resources for Molecular Protocols in Salmonella Research.
520 _aSalmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Because no recent comprehensive literature of this format is available on Salmonella, Salmonella: Methods and Protocols will be of value to a wide variety of researchers engaged in the study of Salmonella, and enterobacteria in general.
700 1 _aSchatten, Heide
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aEisenstark, Abe
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617376740
776 0 8 _iPrinted edition:
_z9781588296191
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-512-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2007
999 _c235556
_d235556