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020 _a9781592599127
024 7 _a10.1385/1592599125
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aProtein'Ligand Interactions
_bMethods and Applications
_cedited by G. Ulrich Nienhaus.
250 _a1st edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XI, 568 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v305
505 0 _aIsothermal Titration Calorimetry -- Direct Optical Detection of Protein-Ligand Interactions -- Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance -- Measurement of Solvent Accessibility at Protein-Protein Interfaces -- Hydrophobic Interaction Chromatography -- Sedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein Interactions -- Protein-Ligand Interaction Probed by Time-Resolved Crystallography -- X-Ray Crystallography of Protein-Ligand Interactions -- Combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins -- NMR Studies of Protein-Ligand Interactions -- Probing Heme Protein-Ligand Interactions by UV/Visible Absorption Spectroscopy -- Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions -- Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy -- Proteins in Motion -- Fluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions -- Ligand Binding With Stopped-Flow Rapid Mixing -- Circular Dichroism Spectroscopy for the Study of Protein-Ligand Interactions -- High-Throughput Screening of Interactions Between G ProteinCoupled Receptors and Ligands Using Confocal Optics Microscopy -- Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics -- Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding -- Atomic Force Microscopy Measurements of Protein-Ligand Interactions on Living Cells -- Computer Simulation of Protein-Ligand Interactions -- Force Probe Molecular Dynamics Simulations -- Study of Ligand-Protein Interactions by Means of Density Functional Theory and First-Principles Molecular Dynamics.
520 _aMolecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions.
700 1 _aNienhaus, G. Ulrich
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617375255
776 0 8 _iPrinted edition:
_z9781588293725
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599125
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2005
999 _c235559
_d235559