000			04078nam a22003615i 4500
001			235560
003			ES-VaUE
005			20221220020712.0
007			cr nn 008mamaa
008			170918s2017 xxu| s |||| 0|eng d
020			_a9781493972654
024	7		_a10.1007/978-1-4939-7265-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSuper-Resolution Microscopy _bMethods and Protocols _cedited by Holger Erfle.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 262 páginas) _b48 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1663
505	0		_aSuper-Resolution Microscopy Techniques and their Potential for Applications in Radiation Biophysics -- Managing the Introduction of Super-Resolution Microscopy into a Core Facility -- Live-Cell STED Imaging with the HyPer2 Biosensor -- Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope -- Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo -- STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells -- Four Channel Super-Resolution Imaging by 3-D Structured Illumination -- Correlative SIM-STORM Microscopy -- Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples -- Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles -- Two-Color Single-Molecule Tracking in Live Cells -- Fully Automated Targeted Confocal and Single Molecule Localization Microscopy -- Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy -- Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells -- Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging Experiments -- Measuring Nanometer Distances between Fluorescent Labels Step-by-Step -- Correlative Single Molecule Localization Microscopy and Confocal Microscopy -- Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells -- In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT -- Super-Resolution High Content Screening and Analysis.
520			_aThis volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.
700	1		_aErfle, Holger _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972647
776	0	8	_iPrinted edition: _z9781493972661
776	0	8	_iPrinted edition: _z9781493984350
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7265-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235560 _d235560