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008			141010s2015 xxu| s |||| 0|eng d
020			_a9781493917556
024	7		_a10.1007/978-1-4939-1755-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNuclear G-Protein Coupled Receptors _bMethods and Protocols _cedited by Bruce G. Allen, Terence E. Hébert.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XV, 215 páginas) _b43 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1234
505	0		_aStudies of Intracellular Angiotensin II -- Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes -- Design and Application of Light-Activated Probes for Cellular Signaling -- Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes -- Quantification of Catecholamine Uptake in Adult Cardiac Myocytes -- Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase --  Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes -- High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs) -- Biochemical Fractionation of Membrane Receptors in the Nucleus -- Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons -- Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors -- Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+] -- Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors -- Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting Proteins -- Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei -- Trafficking and Function of GPCRs in the Endosomal Compartment.
520			_aNuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.
700	1		_aAllen, Bruce G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHébert, Terence E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493917563
776	0	8	_iPrinted edition: _z9781493917549
776	0	8	_iPrinted edition: _z9781493955466
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1755-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235564 _d235564