000			03835nam a22003615i 4500
001			235565
003			ES-VaUE
005			20221220020713.0
007			cr nn 008mamaa
008			170329s2017 xxu| s |||| 0|eng d
020			_a9781493968725
024	7		_a10.1007/978-1-4939-6872-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHuman T-Lymphotropic Viruses _bMethods and Protocols _cedited by Claudio Casoli.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIII, 220 páginas) _b43 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1582
505	0		_aSerological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection -- Molecular Epidemiology Database for Sequence Management and Data Mining -- Reporter Systems to Study HTLV-1 Transmission -- Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells -- Isolation of Exosomes from HTLV-Infected Cells -- A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins -- Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2 -- EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway -- Methods for Identifying and Examining HTLV-1 HBZ Post-Translational Modifications -- High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites -- STR Profiling of HTLV-1-Infected Cell Lines -- Expression of HTLV-1 Genes in T-cells Using RNA Electroporation -- Quantification of Cell Turnover in the Bovine Leukemia Virus Model -- Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection -- Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma.
520			_aWhile HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust methodologies for studying the effects of trans-activating regulatory HTLVs' proteins, the latest techniques for genotyping and gene expression analysis, as well as cellular phenotype and dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Human T-Lymphotropic Viruses: Methods and Protocols serves as an ideal guide to an area of study that is very much worthy of further research.
700	1		_aCasoli, Claudio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968701
776	0	8	_iPrinted edition: _z9781493968718
776	0	8	_iPrinted edition: _z9781493983247
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6872-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235565 _d235565