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001			235576
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008			131111s2014 xxu| s |||| 0|eng d
020			_a9781627037242
024	7		_a10.1007/978-1-62703-724-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Complement System _bMethods and Protocols _cedited by Mihaela Gadjeva.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XVI, 383 páginas) _b81 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1100
505	0		_aOverview -- Evaluation of Complement Function by ELISA -- Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization -- Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional Characterization -- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2 -- Purification and Characterisation of human and Mouse Complement C3 -- Purification of Human Complement Protein C5 -- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry -- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin -- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing -- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System -- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins -- Purification and Functional Analysis of Human Properdin -- Purification and Functional Characterization of C4b-binding Protein (C4BP) -- Purification and Functional Characterization of Factor I -- C1 inhibitor - Quantification and Purification -- Purification, Quantification and Functional Analysis of Complement Factor H -- Factor H-related Proteins -- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS) -- Anti-Factor H Auto Antibodies Assay -- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Functional Analysis of C5a Effector Responses In Vitro and In Vivo -- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry -- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry -- Purification of C1q Receptors and Functional Analysis -- Detection of Cell Membrane-bound CD46 using Flow Cytometry -- Detection of CD59 using Flow Cytometry -- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay -- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry -- Analysis of the Complement Activation in Mice -- Opsonophagocytic Assay.
520			_aComplement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols  to purify and analyze the activity of the individual complement components or pathways.  It includes assays that describe detection  of complement SNPs,  clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established "classical" assays and recently developed "new" assays to analyze the complement system activation will be useful to a wide audience of scientists.
700	1		_aGadjeva, Mihaela _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627037259
776	0	8	_iPrinted edition: _z9781627037235
776	0	8	_iPrinted edition: _z9781493963065
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-724-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235576 _d235576