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001			235577
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008			131206s2014 xxu| s |||| 0|eng d
020			_a9781627037303
024	7		_a10.1007/978-1-62703-730-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTherapeutic Applications of Ribozymes and Riboswitches _bMethods and Protocols _cedited by Daniel Lafontaine, Audrey Dubé.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 278 páginas) _b53 ilustraciones, 19 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1103
505	0		_aIdentification of Regulatory RNA in Bacterial Genomes by Genome-Scale Mapping of Transcription Start Sites -- Screening Inhibitory potential of anti-HIV RT RNA Aptamers -- Design and Evaluation of Clinically Relevant SOFA-HDV Ribozymes Targeting HIV RNA -- Directing RNase P-mediated Cleavage of Target mRNAs by Engineered External Guide Sequences in Cultured Cells -- Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target -- Knockdown Strategies for the Study of Proprotein Converstase and Proliferation in Prostate Cancer Cells -- Use of Tumor-Targeting Trans-Splicing Ribozyme for Cancer Treatment -- Characterization of Hairpin Ribozyme Reactions -- Finding Instances of Riboswitches and Ribozymes by Homology Search of Structured RNA with Infernal -- Structure-based Virtual Screening for the Identification of RNA-binding Ligands -- Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and in-line Probing Assays -- Discovery of Small Molecule Modifiers of microRNAs for the Treatment of HCV Infection -- Bacterial Flavin Mononucleotide Riboswitches as Targets for Flavin Analogs -- Construction and Application of Riboswitch-based Sensors That Detect Metabolites Within Bacterial Cells -- Screening Assays to Identify Artificial glmS Ribozyme Activators -- Analysis of Riboswitch Structure and Ligand Binding using Small-angle X-ray Scattering (SAXS) -- Use of SHAPE to Select 2AP Substitution sites for RNA-ligand Interactions and Dynamics Studies -- Cell Internalization SELEX:  In Vitro Selection for Molecules that Internalize into Cells -- DNA Electronic Switches Based on Analyte-Responsive Aptamers.
520			_aIn Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target.  These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.
700	1		_aLafontaine, Daniel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDubé, Audrey _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627037310
776	0	8	_iPrinted edition: _z9781627037297
776	0	8	_iPrinted edition: _z9781493962969
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-730-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235577 _d235577