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007			cr nn 008mamaa
008			170909s2018 xxu| s |||| 0|eng d
020			_a9781493972340
024	7		_a10.1007/978-1-4939-7234-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aUrothelial Carcinoma _bMethods and Protocols _cedited by Wolfgang A Schulz, Michèle J. Hoffmann, Günter Niegisch.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIV, 362 páginas) _b73 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1655
505	0		_aAnalysis of Chromosomal Alterations in Urothelial Carcinoma -- Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma -- A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer -- Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers -- Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel -- Defining the Pathways of Urogenital Schistosomiasis-associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models -- Algorithm for the Automated Evaluation of NAT2 Genotypes -- Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma -- Oxidative Stress in Urothelial Carcinogenesis: Measurements of Protein Carbonylation and Intracellular Production of Reactive Oxygen Species -- Urothelial Carcinoma Stem Cells - current Concepts, Controversies, and Methods -- In Vitro Differentiation and Propagation of Urothelium from Pluripotent Stem Cell Lines -- Spheroid Cultures of Primary Urothelial Cancer Cells: Cancer Tissue-originated Spheroid (CTOS) Method -- The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model -- Patient-derived Bladder Cancer Xenografts -- Orthotopic Mouse Models of Urothelial Cancer -- Quantification of microRNAs in Urine-derived Specimens -- Quantitative RNA Analysis from Urine using Real Time PCR -- DNA Methylation Analysis from Body Fluids -- Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma -- Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin -- Epigenetic Treatment Options in Urothelial Carcinoma -- Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum -- Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer -- Visualization and Quantitative Measurement of Frug-induced Platinum Sdducts in the Nuclear DNA of Individual Cells by an Immuno-cytological Sssay.
520			_aThis volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis.  Chapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.
700	1		_aSchulz, Wolfgang A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHoffmann, Michèle J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNiegisch, Günter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972333
776	0	8	_iPrinted edition: _z9781493972357
776	0	8	_iPrinted edition: _z9781493984251
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7234-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c235582 _d235582