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020			_a9781592592166
024	7		_a10.1385/1592592163 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSeptic Shock Methods and Protocols _cedited by Thomas J. Evans.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (X, 208 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v36
505	0		_aEndotoxin -- Assay of Endotoxin by Limulus Amebocyte Lysate -- Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria -- Assay of Anti-Endotoxin Antibodies -- Isolation of the Bactericidal/Permeability-Increasing Protein from Polymorphonuclear Leukocytes by Reversible Binding to Target Bacteria -- Purification of Lipopolysaccharide Binding Protein -- Other Bacterial Products -- Purification of Streptococcal Pyrogenic Exotoxin A -- Assay for Superantigens -- Cytokines -- Bioassay for Tumor Necrosis Factors-? and ? -- Assay of Soluble Tumor Necrosis Factor Receptors -- Whole-Blood Assays for Cytokine Production -- Nitric Oxide and Other Reactive Nitrogen Intermediates -- Nitric Oxide Synthase Inhibitors -- In Situ Detection of Nitric Oxide -- Immunochemical Detection of Nitric Oxide Synthase in Human Tissue -- Anti-Nitrotyrosine Antibodies for Immunohistochemistry -- Detection of Peroxynitrite in Biological Fluids -- Cell Culture Techniques -- Myocardial Cells in Culture to Study Effects of Cytokines -- Culture of Primary Human Bronchial Epithelial Cells -- Primary Culture of Human Proximal Renal Tubular Epithelial Cells.
520			_aIn Septic Shock Methods and Protocols, an interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of whole animals-these experienced laboratorians detail the most important techniques for studying sepsis involving endotoxins, toxins derived from gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and the use of primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells. Multidisciplinary and state-of-the-art, Septic Shock Methods and Protocols offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection. Its readily reproducible methods will allow both newcomers and those with more experience to apply today's powerful techniques successfully in their work.
700	1		_aEvans, Thomas J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371547
776	0	8	_iPrinted edition: _z9780896037304
776	0	8	_iPrinted edition: _z9781489941879
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592163 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c235587 _d235587