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020 _a9781597450607
024 7 _a10.1007/978-1-59745-060-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aStem Cells in Regenerative Medicine
_cedited by Julie Audet, William L. Stanford.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XX, 424 páginas)
_b75 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v482
505 0 _aStem Cell Sources and Manipulation -- Derivation and Manipulation of Murine Embryonic Stem Cells -- Patterning Mouse and Human Embryonic Stem Cells Using Micro-contact Printing -- Efficient Gene Knockdowns in Human Embryonic Stem Cells Using Lentiviral-Based RNAi -- Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells -- Stem Cell Sources for Regenerative Medicine -- Regeneration of the Blood System -- Investigating the Interactions Between Haemopoietic Stem Cells and Their Niche: Methods for the Analysis of Stem Cell Homing and Distribution Within the Marrow Following Transplantation -- Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells -- Ex Vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering -- Regeneration of the Nervous System -- Isolation and Manipulation of Mammalian Neural Stem Cells In Vitro -- Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors -- Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Cord -- Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS -- Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys -- Regeneration of the Epidermis -- Isolation and Culture of Epithelial Stem Cells -- Regeneration of Skin and Cornea by Tissue Engineering -- Regeneration of the Musculoskeletal System -- Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone -- Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs) -- Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transduction, and Production of Conditioned Medium -- Template DNA-Strand Co-Segregation and Asymmetric Cell Division in Skeletal Muscle Stem Cells -- Isolation and Grafting of Single Muscle Fibres -- Regeneration of the Vascular System -- Differentiation and Dynamic Analysis of Primitive Vessels from Embryonic Stem Cells -- Derivation of Contractile Smooth Muscle Cells from Embryonic Stem Cells -- Regeneration of the Pancreas and Liver -- Islet-Derived Progenitors as a Source of In Vitro Islet Regeneration -- Isolation and Characterization of Hepatic Stem Cells, or "Oval Cells," from Rat Livers -- Reprogramming of Liver to Pancreas.
520 _aAs regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology™ series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications. .
700 1 _aAudet, Julie
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aStanford, William L
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781603278829
776 0 8 _iPrinted edition:
_z9781617377846
776 0 8 _iPrinted edition:
_z9781588297976
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-060-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c235595
_d235595