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020 _a9781617791826
024 7 _a10.1007/978-1-61779-182-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCell Cycle Synchronization
_bMethods and Protocols
_cedited by Gaspar Banfalvi.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (XII, 300 páginas)
_b57 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v761
505 0 _aOverview of Cell Synchronization -- Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation -- Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells -- Large Scale Mitotic Cell Synchronization -- Synchronization of Mammalian Cell Cultures by Serum Deprivation -- Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry -- Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes -- Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis -- Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover during the Cell Cycle -- Synchronization of HeLa Cells -- Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth -- Synchronization of Yeast -- Synchronization of Pathogenic Protozoans -- Synchronization of In Vitro Maturation in Porcine Oocytes -- Synchronization of Medicago sativa Cell Suspension Culture -- Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT) -- Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation -- Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients -- Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish.
520 _aTo prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.  In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events.  Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the "arrest-and-release" approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Detailed and easy to follow,  Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.
700 1 _aBanfalvi, Gaspar
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617791819
776 0 8 _iPrinted edition:
_z9781617791833
776 0 8 _iPrinted edition:
_z9781493961948
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-182-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c235597
_d235597