000			05951nam a22003615i 4500
001			235599
003			ES-VaUE
005			20221220020715.0
007			cr nn 008mamaa
008			170505s2017 xxu| s |||| 0|eng d
020			_a9781493969555
024	7		_a10.1007/978-1-4939-6955-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aATM Kinase _bMethods and Protocols _cedited by Sergei V. Kozlov.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIV, 437 páginas) _b93 ilustraciones, 69 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1599
505	0		_aAssaying Radiosensitivity of Ataxia-Telangiectasia -- Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology -- ATM Gene Mutation Detection Techniques and Functional Analysis -- An HTRF® Assay for the Protein Kinase ATM -- ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using CellomicsTM ArrayScan VTI Platform -- Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci -- Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy -- Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging -- Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein -- Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry -- Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS) -- Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies -- Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays -- ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry -- Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling -- Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation -- Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics -- ChIP Technique to Study Protein Dynamics at Defined DNA Double-Strand Breaks -- Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System -- Imaging of Fluorescently-Tagged ATM Kinase at the Sites of DNA Double-Strand Breaks -- Live Cell Imaging to Study Real-Time ATM-Mediated Recruitment of DNA Repair Complexes to Sites of Ionizing Radiation-Induced DNA Damage -- Analyzing Heterochromatic DNA Double Strand Break (DSB) Repair in Response to Ionizing Radiation -- Phenotypic Analysis of ATM Protein Kinase in DNA Double-Strand Break Formation and Repair -- Monitoring DNA Repair Consequences of ATM Signaling Using Simultaneous Fluorescent Readouts -- Non-Canonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage -- Study of ATM Phosphorylation by Cdk5 in Neuronal Cells -- DNA Damage Response in Human Stem Cells and Neural Descendants -- A Patient-Specific Stem Cell Model to Investigate the Neurological Phenotype Observed in Ataxia-Telangiectasia -- Lentiviral Reprogramming of A-T Patient Fibroblasts to Induced Pluripotent Stem Cells -- Monitoring the ATM-Mediated DNA Damage Response in the Cerebellum Using Organotypic Cultures.
520			_aAtaxia-telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successfulMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.
700	1		_aKozlov, Sergei V _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969531
776	0	8	_iPrinted edition: _z9781493969548
776	0	8	_iPrinted edition: _z9781493983506
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6955-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235599 _d235599