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020 _a9781592599554
024 7 _a10.1385/1592599559
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aNatural Products Isolation
_cedited by Satya D. Sarker.
250 _a2nd edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XII, 516 páginas)
_b133 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Biotechnology
_x1940-607X
_v20
505 0 _aNatural Product Isolation -- Initial and Bulk Extraction -- Supercritical Fluid Extraction -- An Introduction to Planar Chromatography -- Isolation of Natural Products by Low-Pressure Column Chromatography -- Isolation by Ion-Exchange Methods -- Separation by High-Speed Countercurrent Chromatography -- Isolation by Preparative High-Performance Liquid Chromatography -- Hyphenated Techniques -- Purification by Solvent Extraction Using Partition Coefficient -- Crystallization in Final Stages of Purification -- Dereplication and Partial Identification of Compounds -- Extraction of Plant Secondary Metabolites -- Isolation of Marine Natural Products -- Isolation of Microbial Natural Products -- Purification of Water-Soluble Natural Products -- Scale-Up of Natural Product Isolation -- Follow-Up of Natural Product Isolation.
520 _aBuilding on the high reputation of the first edition of Natural Products Isolation, the authors have in this second edition not only significantly updated all the techniques to reflect recent advances in the field, but also added three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. These readily reproducible methods make it possible for even the novice to extract, prepare, and isolate natural products from given source materials. The book includes not only powerful cutting-edge techniques, but also simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Natural Products Isolation, Second Edition, offers the aspiring natural products researcher all the information needed to attain mastery of essential methods and the more experienced investigator a useful reference guide to all the available techniques.
700 1 _aSarker, Satya D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617375750
776 0 8 _iPrinted edition:
_z9781588294470
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599559
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2005
999 _c235602
_d235602