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008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597453219
024	7		_a10.1007/978-1-59745-321-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicrobial Gene Essentiality: Protocols and Bioinformatics _cedited by Andrei L. Osterman, Svetlana Y. Gerdes.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XIV, 486 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v416
505	0		_aOverview of Whole-Genome Essentiality Analysis -- Experimental Protocols -- Transposon-Based Strategies for the Identification of Essential Bacterial Genes -- Identification and Analysis of Essential Genes in Haemophilus influenzae -- Transposon Site Hybridization in Mycobacterium tuberculosis -- Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis -- Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting -- Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis -- Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast -- How to Make a Defined Near-Saturation Mutant Library. Case 1: Pseudomonas aeruginosa PAO1 -- Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality -- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12 -- The Applications of Systematic In-Frame, Single-Gene Knockout Mutant Collection of Escherichia coli K-12 -- A Novel, Simple, High-Throughput Method for Isolation of Genome-Wide Transposon Insertion Mutants of Escherichia coli K-12 -- High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains -- Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray -- Scarless Engineering of the Escherichia coli Genome -- Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System -- Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome -- Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library -- Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus -- of Conditional Lethal Amber Mutations in Escherichia coli -- Bioinformatics -- Statistical Methods for Building Random Transposon Mutagenesis Libraries -- Statistical Evaluation of Genetic Footprinting Data -- Modeling Competitive Outgrowth of Mutant Populations: Why Do Essentiality Screens Yield Divergent Results? -- Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays -- Profiling of Escherichia coli Chromosome Database -- Gene Essentiality Analysis Based on DEG, a Database of Essential Genes -- Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis -- Design and Application of Genome-Scale Reconstructed Metabolic Models -- Predicting Gene Essentiality Using Genome-Scale in Silico Models -- Comparative Approach to Analysis of Gene Essentiality.
520			_aMicrobial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.
700	1		_aOsterman, Andrei L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGerdes, Svetlana Y _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375293
776	0	8	_iPrinted edition: _z9781588293787
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-321-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235603 _d235603