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008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597455831
024	7		_a10.1007/978-1-59745-583-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDrosophila _bMethods and Protocols _cedited by Christian Dahmann.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XII, 438 páginas) _b101 ilustraciones, 13 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v420
505	0		_aDrosophila melanogaster and the Development of Biology in the 20th Century -- Getting Started -- FlyBase -- The Use of P-Element Transposons to Generate Transgenic Flies -- The GAL4 System -- P-Element Mutagenesis -- EMS Screens -- RNAi Screening in Cultured Drosophila Cells -- Methods for Homologous Recombination in Drosophila -- Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis -- Cuticle Preparation of Drosophila Embryos and Larvae -- Immunolabeling of Embryos -- Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins -- Analysis of Neuromuscular Junctions -- Immunolabeling of Imaginal Discs -- Imaging Drosophila Pupal Wing Morphogenesis -- Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections -- Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues -- Linear RNA Amplification for the Production of Microarray Hybridization Probes -- An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs -- Extraction and Immunoblotting of Proteins From Embryos -- Purification of Drosophila Protein Complexes for Mass Spectrometry -- Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes -- Flow Cytometric Analysis of Drosophila Cells -- Drosophila Cell Lines as Model Systems and as an Experimental Tool.
520			_aWith its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In Drosophila: Methods and Protocols, leading experts contribute a collection of protocols covering standard techniques as well as novel methods. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters contain brief introductions to the subject matter, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and essential, Drosophila: Methods and Protocols allows novices and experts alike to tap into the power of one hundred years of expertise involving this vital model system.
700	1		_aDahmann, Christian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617377914
776	0	8	_iPrinted edition: _z9781588298171
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-583-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235606 _d235606