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001			235609
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008			161011s2017 xxu| s |||| 0|eng d
020			_a9781493965335
024	7		_a10.1007/978-1-4939-6533-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aIsolation of Plant Organelles and Structures _bMethods and Protocols _cedited by Nicolas L. Taylor, A. Harvey Millar.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 338 páginas) _b51 ilustraciones, 29 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1511
505	0		_aThe Isolation of Plant Organelles and Structures in the Post-Genomics Era -- Approaches to Characterize Organelle, Compartment, or Structure Purity -- Isolation of Nuclei and Nucleoli -- Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts -- Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit -- Leucoplast Isolation and Subfractionation -- Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes -- Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane -- Isolation of Vacuoles and the Tonoplast -- Isolation of Endoplasmic Reticulum and Its Membrane -- Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings -- Isolation of Autolysosomes from Tobacco BY-2 Cells -- Isolation of Protein Storage Vacuoles and Their Membranes -- Isolation of the Cell Wall -- Isolation of Plasmodesmata -- Isolation of Plasma Membrane and Plasma Membrane Microdomains -- Enrichment of the Plant Cytosolic Fraction -- Isolation of Apoplast -- Isolation of Cytosolic Ribosomes -- Isolation of Plastid Ribosomes -- Isolation of Mitochondrial Ribosomes -- Isolation of Microtubules and Microtubule-Associated Proteins -- Isolation of Actin and Actin Binding Proteins -- Purification of 26S Proteasomes and Their Sub-Complexes from Plants.
520			_aThis book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.
700	1		_aTaylor, Nicolas L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMillar, A. Harvey _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493965311
776	0	8	_iPrinted edition: _z9781493965328
776	0	8	_iPrinted edition: _z9781493982288
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6533-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235609 _d235609