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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592388
024	7		_a10.1385/1592592384 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGene Probes _bPrinciples and Protocols _cedited by Marilena Aquino do Muro, Ralph Rapley.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XI, 272 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v179
505	0		_aTarget Format and Hybridization Conditions -- End Labeling Procedures -- Photobiotin Labeling -- Nick Translation -- Random Primed Labeling -- Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization -- Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors -- The ?-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites -- Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma -- Detection of Chlamydia trachomatis by DNA Amplification -- Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes -- Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction -- Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes -- Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4 -- Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) -- Analysis of 16S-23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species -- Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes -- In Situ Hybridization of Burkholderia cepacia Cells -- Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil -- Detection of Single-Copy Genes in DNA from Transgenic Plants -- Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission -- Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food -- Polymerase Chain Reaction for Detection of Listeria monocytogenes.
520			_aThe use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today.
700	1		_aAquino do Muro, Marilena _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372360
776	0	8	_iPrinted edition: _z9780896038851
776	0	8	_iPrinted edition: _z9781489938978
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592384 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235615 _d235615