000 06214nam a22003735i 4500
001 235616
003 ES-VaUE
005 20221220020716.0
007 cr nn 008mamaa
008 120211s2012 xxu| s |||| 0|eng d
020 _a9781617795398
024 7 _a10.1007/978-1-61779-539-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHost-Fungus Interactions
_bMethods and Protocols
_cedited by Alexandra C. Brand, Donna M. MacCallum.
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XVI, 597 páginas)
_b104 ilustraciones, 37 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v845
505 0 _aGene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy -- Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans -- Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans -- Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi -- Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers -- Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System -- Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast -- Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach -- Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers -- RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum -- RNA Interference in Cryptococcus neoformans -- Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA -- Tetracycline-Inducible Gene Expression in Candida albicans -- Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii -- Modular Gene Over-Expression Strategies for Candida albicans -- Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans -- Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans -- Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils -- Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions -- Simple Assays for Measuring Innate Interactions with Fungi -- Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells -- Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium -- Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR -- In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus -- Biofilm Formation Studies in Microtiter Plate Format -- Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of Infection -- Laser Capture Microdissection of Candida albicans from Host Tissue -- Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples -- Cytokine Measurement Using Cytometric Bead Arrays (CBA) -- Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis -- Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi -- Drosophila melanogaster as a Model Organism for Invasive Aspergillosis -- Galleria mellonella as a Model for Fungal Pathogenicity Testing -- Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi -- Mouse Intravenous Challenge Models and Applications -- A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis -- Invasive Models of Histoplasmosis -- Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization -- A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections -- Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter -- Orogastrointestinal Model of Mucosal and Disseminated Candidiasis -- A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis.
520 _aMicrobiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.  Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.
700 1 _aBrand, Alexandra C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMacCallum, Donna M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617795381
776 0 8 _iPrinted edition:
_z9781617795404
776 0 8 _iPrinted edition:
_z9781493959044
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-539-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2012
999 _c235616
_d235616