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020 _a9781597450379
024 7 _a10.1385/1597450375
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aEmbryonic Stem Cell Protocols
_bVolume I: Isolation and Characterization
_cedited by Kursad Turksen.
250 _a2nd edition 2006
264 1 _aTotowa, NJ
_bHumana Press
_c2006
300 _a1 recurso en línea (536 páginas)
_b216 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v329
505 0 _aIsolation and Maintenance -- Isolation and Differentiation of Medaka Embryonic Stem Cells -- Maintenance of Chicken Embryonic Stem Cells In Vitro -- Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro -- Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro -- Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells -- Generation and Characterization of Monkey Embryonic Stem Cells -- Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture -- Signaling in Embryonic Stem Cell Differentiation -- Internal Standards in Differentiating Embryonic Stem Cells In Vitro -- Matrix Assembly, Cell Polarization, and Cell Survival -- Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells -- Cripto Signaling in Differentiating Embryonic Stem Cells -- The Use of Embryonic Stem Cells to Study Hedgehog Signaling -- Transfection and Promoter Analysis in Embryonic Stem Cells -- SAGE Analysis to Identify Embryonic Stem Cell-Predominant Transcripts -- Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4 -- Gene Silencing Using RNA Interference in Embryonic Stem Cells -- Genetic Manipulation of Embryonic Stem Cells -- Efficient Transfer of HSV-1 Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives -- Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells -- Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expression in Mouse Embryonic Stem Cells -- Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells -- Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells -- DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells -- Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro -- Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens -- Use of Differentiating Embryonic Stem Cells in Pharmacological Studies -- Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens -- Use of Murine Embryonic Stem Cells in Embryotoxicity Assays -- Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells -- Epigenetic Analysis of Embryonic Stem Cells -- Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion -- Methylation in Embryonic Stem Cells In Vitro -- Tumor-Like Properties -- Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells -- In Vivo Tumor Formation From Primate Embryonic Stem Cells -- Animal Models and Therapy -- Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Therapy -- Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model.
520 _aDrawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.
700 1 _aTurksen, Kursad
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617376016
776 0 8 _iPrinted edition:
_z9781588294982
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597450375
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2006
999 _c235618
_d235618