000			03063nam a22003495i 4500
001			235624
003			ES-VaUE
005			20221220020716.0
007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597452984
024	7		_a10.1007/978-1-59745-298-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSingle Cell Diagnostics _bMethods and Protocols _cedited by Alan R. Thornhill.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XI, 182 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v132
505	0		_aNoninvasive Metabolic Assessment of Single Cells -- Laser Capture Microdissection for Analysis of Single Cells -- Fluorescence In Situ Hybridization on Single Cells -- Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis -- Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma -- Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification -- Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies -- Efficient Isothermal Amplification of the Entire Genome from Single Cells -- Comparative Genomic Hybridization on Single Cells -- Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells -- Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells -- Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization -- Microarray Technology for Mutation Analysis of Low-Template DNA Samples.
520			_aThe aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
700	1		_aThornhill, Alan R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617376542
776	0	8	_iPrinted edition: _z9781588295781
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-298-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c235624 _d235624