000			03101nam a22003495i 4500
001			235630
003			ES-VaUE
005			20221220020717.0
007			cr nn 008mamaa
008			100301s1997 xxu| s |||| 0|eng d
020			_a9781592595570
024	7		_a10.1385/0896033953 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPRINS and In Situ PCR Protocols _cedited by John R. Gosden.
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XIII, 165 páginas) _b75 ilustraciones, 22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v71
505	0		_aOligonucleotide PRINS DNA Synthesis -- Chromosome-Specific PRINS -- Bright-Field Microscopic Detection of Oligonucleotide PRINS-Labeled DNA in Chromosome Preparations -- Analysis of Sperm Aneuploidy by PRINS -- PRINS DNA Synthesis on Frozen Tissue Sections -- Multiple Sequential Oligonucleotide Primed In Situ DNA Syntheses (MULTI-PRINS) -- Oligonucleotide PRINS DNA Synthesis on Extended Chromatin Preparations -- Combined lmmunocytochemistry and PRINS DNA Synthesis for Simultaneous Detection of Phenotypic and Genomic Parameters in Cells -- Chromosomal PRINS DNA Labeling Combined with Indirect Immunocytochemistry -- Direct In Situ Single-Copy (DISC)-PCR -- In Situ PCR to Cells and to Wax Sections -- Reverse Transcriptase PCR In Situ on Cryopreserved Tissue Sections -- Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA -- In Situ PCR Amplification of Intracellular mRNA -- Localized In Situ Amplification (LISA) -- In Situ PCR.
520			_aCutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.
700	1		_aGosden, John R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9780896033955
776	0	8	_iPrinted edition: _z9781489928009
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033953 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1997
999			_c235630 _d235630