000			03781nam a22003495i 4500
001			235632
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598731
024	7		_a10.1385/1592598730 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aImmunochemical Protocols _cedited by Robert Burns.
250			_a3rd edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (X, 318 páginas) _b22 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v295
505	0		_aImmunization Strategies for Antibody Production -- Synthetic Peptides as Antigens for Antibody Production -- Production of Polyclonal Antibodies in Rabbits -- Making Hybridomas -- Growing Hybridomas -- Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments -- Purification of Antibodies and Preparation of Antibody Fragments -- Conjugation of Antibodies to Alkaline Phosphatase -- Conjugation of Antibodies to Horseradish Peroxidase -- Conjugation of Antibodies to Fluorescein or Rhodamine -- Conjugation of Antibodies to Biotin -- Preparation of Colloidal Gold Probes -- Immunogold Probes in Light Microscopy -- Immunoelectron Microscope Techniques in Plant Virus Diagnosis -- Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay -- Competitive Enzyme-Linked Immunosorbent Assay -- Immunoblotting Techniques -- Epitope Mapping -- Generation and Testing of Immunoaffinity Columns -- Immunocapture-Polymerase Chain Reaction -- Magnetic Cell Sorting -- Cell Enrichment and Immunochemical Staining.
520			_aThis new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes.
700	1		_aBurns, Robert _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617374661
776	0	8	_iPrinted edition: _z9781588292742
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598730 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c235632 _d235632