000			03320nam a22003495i 4500
001			235639
003			ES-VaUE
005			20221220020717.0
007			cr nn 008mamaa
008			100301s1988 xxu| s |||| 0|eng d
020			_a9781592594900
024	7		_a10.1385/0896031268 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNew Protein Techniques _cedited by John M. Walker.
250			_a1st edition 1988
264		1	_aTotowa, NJ _bHumana Press _c1988
300			_a1 recurso en línea (XV, 531 páginas) _b33 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v3
505	0		_aPrevention of Unwanted Proteolysis -- The Bradford Method for Protein Quantitation -- Amino Acid Analysis by Precolumn Derivatization -- Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Design, Preparation, and Use of Immunopurification Reagents -- Dye-Ligand Chromatography -- Aminohexyl-Sepharose Affinity Chromatography -- Purification of DNA-Dependent RNA Polymerase from Eubacteria -- Direct Immunoprecipitation of Protein -- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Chromatofocusing -- Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels -- Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Preparative Aspects of Immobilized pH Gradients -- Isoelectric Focusing Under Denaturing Conditions -- Computer Analysis of 2-D Electrophoresis Gels -- Two-Dimensional (Crossed) Immunoelectrophoresis -- Peptide Synthesis -- Synthesis of a Series of Analogous Peptides Using T-Bags -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Purification of Immunoglobulins Using Protein A-Sepharose -- Antibody-Enzyme Conjugate Formation -- Vacuum Blotting -- Blotting with Plate Electrodes -- Use of Dried Milk for Immunoblotting -- Immunodetection of Proteins on "Western" Blots Using 125I-Labeled Protein A -- Detection of Protein Blots Using the Avidin-Biotin System -- Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A -- Colloidal Gold for the Detection of Proteins on Blots and Immunoblots -- Enzyme Immobilization by Adsorption -- Enzyme Immobilization by Entrapment -- Enzyme Immobilization by Covalent Bonding -- Cell Immobilization.
700	1		_aWalker, John M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943811
776	0	8	_iPrinted edition: _z9781489943804
776	0	8	_iPrinted edition: _z9780896031265
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896031268 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1988
999			_c235639 _d235639