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008			130916s2009 xxu| s |||| 0|eng d
020			_a9781597452014
024	7		_a10.1007/978-1-59745-201-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHepatocyte Transplantation _bMethods and Protocols _cedited by Anil Dhawan, Robin D. Hughes.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVII, 231 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v481
505	0		_aHuman Hepatocyte Transplantation Overview -- Isolation of Human Hepatocytes -- An Optimised Method for Cryopreservation of Human Hepatocytes -- Liver Cell Culture Techniques -- In vitro Assays for Induction of Drug Metabolism -- Hepatocyte Apoptosis -- Small Animal Models of Hepatocyte Transplantation -- Hepatocyte Transplantation Techniques: Large Animal Models -- Cell Transplant Techniques: Engraftment Detection of Cells -- Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation -- Ex vivo Gene Transfer into Hepatocytes -- Sources of Adult Hepatic Stem Cells: Haematopoietic -- Production of Hepatocyte-like Cells from Human Amnion -- Generation of Hepatocytes from Human Embryonic Stem Cells -- Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells -- Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro -- Liver Cell Labelling with MRI Contrast Agents -- Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory.
520			_aDue to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
700	1		_aDhawan, Anil _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHughes, Robin D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607611721
776	0	8	_iPrinted edition: _z9781627037532
776	0	8	_iPrinted edition: _z9781588298836
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-201-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235642 _d235642