000			04110nam a22003495i 4500
001			235643
003			ES-VaUE
005			20221220020717.0
007			cr nn 008mamaa
008			100301s1994 xxu| s |||| 0|eng d
020			_a9781592595198
024	7		_a10.1385/089603268X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBasic Protein and Peptide Protocols _cedited by John M. Walker.
250			_a1st edition 1994
264		1	_aTotowa, NJ _bHumana Press _c1994
300			_a1 recurso en línea (XII, 490 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v32
505	0		_aThe Lowry Method for Protein Quantitation -- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation -- The Bradford Method for Protein Quantitation -- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins -- SDS Polyacrylamide Gel Electrophoresis of Proteins -- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins -- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins -- Electrophoresis of Peptides -- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels -- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive) -- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Identification of Glycoproteins on Nitrocellulose Membranes and Gels -- The Release of Oligosaccharides from Glycoproteins -- Structural Profiling of Oligosaccharides of Glycoproteins -- Drying Gels -- Fluorography of Polyacrylamide Gels Containing Tritium -- The Electrophoretic Elution of Proteins from Polyacrylamide Gels -- Preparation of Proteins from Gels for Protein Microsequencing -- Comparison of Protein Primary Structures -- Protein Blotting -- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A -- Detection of Proteins on Blots Using the Avidin-Biotin System -- Detection of Proteins on Protein Blots Using Chemiluminescent Systems -- The Detection of Proteins on Blots Using Gold or Immunogold -- Amino Acid Analysis -- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography -- Peptide Purification by Reverse-Phase HPLC -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Dansyl Method for Identifying N-Terminal Amino Acids -- The Dansyl-Edman Method for Peptide Sequencing -- C-Terminal Sequencing of Peptides -- Analysis of Cysteine Residues and Disulfide Bonds -- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components -- The Raising of a Polyclonal Antiserum to a Protein -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Preparation of Purified Immunoglobulin G (IgG) -- Enzyme and Biotin Labeling of Antibody -- Labeling of Peptides and Proteins by Radioiodination -- Radioimmunoassay of Peptides and Proteins -- Enzyme-Linked Immunosorbent Assay (ELISA) -- Free Zone Capillary Electrophoresis.
700	1		_aWalker, John M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9780896032699
776	0	8	_iPrinted edition: _z9781489939852
776	0	8	_iPrinted edition: _z9780896032682
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/089603268X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1994
999			_c235643 _d235643