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001			235644
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007			cr nn 008mamaa
008			150929s2015 xxu| s |||| 0|eng d
020			_a9781493929634
024	7		_a10.1007/978-1-4939-2963-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aImmunosenescence _bMethods and Protocols _cedited by Albert C. Shaw.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 270 páginas) _b49 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1343
505	0		_aIsolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation -- Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing -- Flow Cytometric Identification of Fibrocytes in the Human Circulation -- Experimental Approaches to Tissue Injury and Repair in Advanced Age -- Multicolor Digital Flow Cytometry Used in Human Translational Immunology -- Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates -- Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry -- Imaging Immunosenescence -- Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine -- Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry -- Cell-Mediated Immune Response to Influenza Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses -- Assays for Monitoring Macroautophagy Activity in T Cells -- Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues -- Monitoring the DNA Damage Response at Dysfunctional Telomeres -- Single-Cell Analysis of T Cell Receptor ab Repertoire -- Assessment of B Cell Repertoire in Humans -- Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing -- Discovery of Novel MicroRNAs in Aging C. Elegans -- Analysis of DNA Methylation by Pyrosequencing.
520			_aIn this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire.  In addition,  Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.
700	1		_aShaw, Albert C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929627
776	0	8	_iPrinted edition: _z9781493929641
776	0	8	_iPrinted edition: _z9781493944187
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2963-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235644 _d235644