000			03921nam a22003735i 4500
001			235651
003			ES-VaUE
005			20221220020718.0
007			cr nn 008mamaa
008			160520s2016 xxu| s |||| 0|eng d
020			_a9781493936649
024	7		_a10.1007/978-1-4939-3664-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGap Junction Protocols _cedited by Mathieu Vinken, Scott R. Johnstone.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIII, 235 páginas) _b45 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1437
505	0		_aAnalysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction -- DNA Methylation Analysis of Human Tissue-Specific Connexin Genes -- Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis -- Immunohisto- and Cytochemistry Analysis of Connexins -- Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells -- Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor -- Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells -- Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis -- Scrape Loading/Dye Transfer Assay -- Microinjection Technique for Assessment of Gap Junction Function -- Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling -- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro -- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging -- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells -- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels -- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
520			_aPresenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.
700	1		_aVinken, Mathieu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aJohnstone, Scott R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936625
776	0	8	_iPrinted edition: _z9781493936632
776	0	8	_iPrinted edition: _z9781493981106
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3664-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235651 _d235651