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020 _a9781592592500
024 7 _a10.1385/1592592503
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCalcium Signaling Protocols
_cedited by David G. Lambert.
250 _a1st edition 1999
264 1 _aTotowa, NJ
_bHumana Press
_c1999
300 _a1 recurso en línea (XIII, 359 páginas)
_b63 ilustraciones, 2 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v114
505 0 _aGeneral -- Fluorescent Measurement of [Ca2+]c -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1 -- Specialist Measurement Systems -- Confocal Microscopy -- Measurement of Intracellular Calcium Concentration Using Confocal Microscopy -- Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) -- Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) -- Nonelectrophysiological measurement of Ca2+ channel activity -- Measurement of Ca2+ Entry Using 45Ca2+ -- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding -- Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores -- Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay -- Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells -- Photolysis of Caged Calcium Using a Low-Cost Flash Unit -- Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) -- Continuous Fluorescent Monitoring of Cellular Calcium Fluxe -- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators -- Specialist Measurement Techniques -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Measurement of Calcium and Movement in Heart Cells -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Ca2+ Sensitive Targets -- Assay and Purification of Calmodulin-Dependent Protein Kinase -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).
520 _aIn Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents.
700 1 _aLambert, David G
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489943200
776 0 8 _iPrinted edition:
_z9781489943194
776 0 8 _iPrinted edition:
_z9780896035973
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592503
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1999
999 _c235652
_d235652