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008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598700
024	7		_a10.1385/1592598706 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMedical BioMethods Handbook _cedited by John M. Walker, Ralph Rapley.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XVI, 644 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aBasic Techniques in Molecular Biology -- Probe Design, Production, and Applications -- Restriction Enzymes -- Southern Blotting as a Diagnostic Method -- Western Blotting as a Diagnostic Method -- Principles and Medical Applications of the Polymerase Chain Reaction -- Single-Strand Conformation Polymorphism (SSCP) Analysis -- Denaturing Gradient Gel Electrophoresis (DGGE) -- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing -- to Capillary Electrophoresis of DNA -- Mapping Techniques -- Dideoxyfingerprinting for Mutation Detection -- Conformation-Sensitive Gel Electrophoresis -- Amplification Refractory Mutation System and Molecular Diagnostics -- Ligase Chain Reaction -- Chemical Cleavage of Mismatch -- The Protein Truncation Test -- Linkage, Allele Sharing, and Association -- Single-Nucleotide Polymorphisms -- cDNA Microarrays -- Nucleic Acid Sequence-Based Amplification -- Oligonucleotide Ligation Assay -- Quantitative TaqMan Real-Time PCR -- Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine -- Quantitative PCR -- Liquid Chromatography-Mass Spectrometry of Nucleic Acids -- Comparative Genomic Hybridization in Clinical and Medical Research -- Bioinformatic Tools for Gene and Protein Sequence Analysis -- In SituHybridization -- Enzyme-Linked Immunosorbent Assay -- Protein Therapeutics -- Karyotyping -- Microsatellite Analysis -- Analysis of Chromosomal Translocations -- Differential Display -- Techniques for Gene Expression Profiling -- Capillary Electrophoresis in Clinical Analysis -- Flow Cytometry in the Biomedical Arena -- Immunocytochemistry -- Ribotyping in Clinical Microbiology -- Diagnostic Applications of Protein Microarrays -- The Human Genome Project -- Prenatal Diagnosis of Inborn Errors of Metabolism -- Gene Therapy.
520			_aPowerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today. .
700	1		_aWalker, John M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781588292889
776	0	8	_iPrinted edition: _z9781588293343
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598706 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c235665 _d235665