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020 _a9781627033893
024 7 _a10.1007/978-1-62703-389-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMicrosatellites
_bMethods and Protocols
_cedited by Stella K. Kantartzi.
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XI, 339 páginas)
_b71 ilustraciones, 43 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1006
505 0 _aMicrosatellites: Evolution and Contribution -- Screening of Genomic Libraries -- PCR-Based Identification of Microsatellite Arrays (PIMA) -- Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) -- Microsatellite DNA Capture from Enriched Libraries -- Next-Generation Sequencing for High-Throughput Molecular Ecology: A Step-by-Step Protocol for Targeted Multilocus Genotyping by Pyrosequencing -- Optimizing Selection of Microsatellite Loci from 454 Pyrosequencing Via Post-Sequencing Bioinformatic Analyses -- Identification of DNA-Microsatellite Markers for the Characterization of Somatic Embryos in Quercus suber -- Simple Sequence Repeats (SSR) Amplification -- Microsatellite Amplification in Plants: Optimization Procedure of Major PCR Components -- Development of a Multiplex PCR Assay for Characterization of Embryonic Stem Cells -- Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis for Visualization of Single Sequence Repeats -- Microsatellite Fragment Analysis Using the ABI Prism® 377 DNA Sequencer -- Robust and Inexpensive SSR Markers Analyses Using LI-COR DNA Analyzer -- The Use of the MegaBACE for Sequencing and Genotype Analysis -- Analyzing Microsatellites Using the QIAxcel System -- Microsatellite Analysis of Malaria Parasites -- Informativeness of Microsatellite Markers -- Microsatellite Data Analysis for Population Genetics -- Molecular Mapping and Breeding with Microsatellite Markers.
520 _aMicrosatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems.  Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research.  Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.
700 1 _aKantartzi, Stella K
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627033909
776 0 8 _iPrinted edition:
_z9781627033886
776 0 8 _iPrinted edition:
_z9781493963096
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-389-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2013
999 _c235666
_d235666