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008			100301s2001 xxu| s |||| 0|eng d
020			_a9781592591398
024	7		_a10.1385/1592591396 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNonviral Vectors for Gene Therapy _bMethods and Protocols _cedited by Mark A. Findeis.
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XI, 399 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v65
505	0		_aSynthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier -- Cationic ?-Helical Peptides for Gene Delivery into Cells -- Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (L-lysine) Dendrimer with Plasmid DNA -- Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery -- Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers -- Use of Disulfide Cationic Lipids in Plasmid DNA Delivery -- Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein -- Coupling of Nuclear Localization Signals to Plasmid DNA -- Progress Toward a Synthetic Virus -- Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers -- Methods for Studying the Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery -- Characterization of DNA Condensates by Atomic Force Microscopy -- Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting -- Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA -- Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes -- Nonviral DNA Delivery from Polymeric Systems -- Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers -- Lyophilization of Nonviral Gene Delivery Systems -- Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes -- Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy -- Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry -- Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors -- Renal Gene Therapy.
520			_aNonviral gene therapy-an emerging field with great clinical promise-avoids many problems associated with viral gene therapy and offers the possibility of superior clinical effectiveness. In Nonviral Vectors for Gene Therapy: Methods and Protocols, Mark A. Findeis has assembled a panel of active researchers to present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact complexes that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents. Opportunities for the development and application of these novel vectors in the research lab and eventually, in the clinic, are highlighted. Comprehensive and state-of-the-art, Nonviral Vectors for Gene Therapy: Methods and Protocols illuminates for today's investigators the powerful new approaches to the creation of nonviral materials, as well as their efficacious use in delivering therapeutic genes to patients across the spectrum of human disease.
700	1		_aFindeis, Mark A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371455
776	0	8	_iPrinted edition: _z9780896037120
776	0	8	_iPrinted edition: _z9781489942012
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591396 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2001
999			_c235677 _d235677