000			03819nam a22003735i 4500
001			235681
003			ES-VaUE
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007			cr nn 008mamaa
008			130525s2013 xxu| s |||| 0|eng d
020			_a9781627034593
024	7		_a10.1007/978-1-62703-459-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGuanylate Cyclase and Cyclic GMP _bMethods and Protocols _cedited by Thomas Krieg, Robert Lukowski.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 249 páginas) _b46 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1020
505	0		_aNO-cGMP: The Past, the Present and the Future -- cGMP Dependent Protein Kinases (cGK) -- Enzyme Assays for cGMP Hydrolysing Phosphodiesterases -- Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues -- Hyperspectral Imaging of FRET-based cGMP Probes -- Visualization of cGMP with cGi Biosensors -- Advances and techniques to measure cGMP in intact cardiomyocytes -- Real-time Monitoring the Spatio-temporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells -- Methods for Identification of cGKI Substrates -- Approaches for Monitoring PKG1a Oxidative Activation -- Analysis of cGMP Signaling in Adipocytes -- A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signalling Mutant Mice -- Receptor Binding Assay for NO-Independent Activators of soluble Guanylate Cyclase -- Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing -- The Geisler Method: Tracing Activity Dependent cGMP Plasticity Changes Upon Double Detection of mRNA and Protein on Brain Slices -- Detection of cGMP in the Degenerating Retina.
520			_aRecently, many ground-breaking steps have been made towards  better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.       Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide  scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.
700	1		_aKrieg, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLukowski, Robert _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627034609
776	0	8	_iPrinted edition: _z9781627034586
776	0	8	_iPrinted edition: _z9781493963201
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-459-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235681 _d235681